Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids.
Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, Durnberger G, Mechtler K, Knoblich JA
Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196190 | pSpCas9(BB)-2A-tomato SOX2-sgRNA | Editing human SOX2 locus |
| 196191 | pHDR_sox2HA_P2A-GFP-pA_HA_G2913A | Tagging human SOX2 gene with P2A-EGFP at the C-terminus |
| 196192 | AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA | human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus |
| 196195 | pSpCas9(BB)-2A-tomato-TSC2-sgRNA | Editing human TSC2 locus |
| 196196 | pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro | homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus |
| 196197 | pSpCas9(BB)-2A-Puro G3BP1-sgRNA | Cas9 + gRNA expression to edit G3BP1 locus |