Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity.

Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, Beckham GT, McGeehan JE
Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
176762	pEE005	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176763	pEE006	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176764	pEE007	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176765	pEE008	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176766	pEE009	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176767	pEE010	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176768	pEE011	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176769	pEE012	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176770	pEE013	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176771	pEE014	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176772	pEE015	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176773	pEE016	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176774	pEE017	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176775	pEE018	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176776	pEE019	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176777	pEE020	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176778	pEE021	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176779	pEE022	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176780	pEE023	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176781	pEE024	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176782	pEE025	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176783	pEE026	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176784	pEE027	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176785	pEE028	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176786	pEE029	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176787	pEE030	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176788	pEE031	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176789	pEE032	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176790	pEE033	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176791	pEE034	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176792	pEE035	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176793	pEE036	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176794	pEE037	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176795	pEE038	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176796	pEE039	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176797	pEE040	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176798	pEE041	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176799	pEE042	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176800	pEE043	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176801	pEE044	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176802	pEE045	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176803	pEE046	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176804	pEE047	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176805	pEE048	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176806	pEE049	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176807	pEE050	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176808	pEE051	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176809	pEE052	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176810	pEE053	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176811	pEE054	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176812	pEE055	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176813	pEE056	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176814	pEE057	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176815	pEE058	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176816	pEE059	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176817	pEE060	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176818	pEE061	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176819	pEE062	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176820	pEE063	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176821	pEE064	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176822	pEE065	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176823	pEE066	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176824	pEE067	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176825	pEE068	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176826	pEE069	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176827	pEE070	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176828	pEE071	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176829	pEE072	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176830	pEE073	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176831	pEE074	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176832	pEE075	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176833	pEE076	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176834	pEE077	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
176835	pEE078	Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression