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pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.

Maestroni L, Butti P, Senatore VG, Branduardi P
FEMS Yeast Res. 2023 Jan 4;23:foad002. doi: 10.1093/femsyr/foad002. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196040pCEC-redAllows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

Antibodies from Article