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An integrase toolbox to record gene-expression during plant development.

Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
195885L0-T7 Empty target Tp901Level 0 Empty Tp901 target: attP_Tp901-BpiI-attB_Tp901 with golden gate linker in pBLUNT plasmid.
195886L0-T3 mTurq-tUBQ10Level 0 mTurq-tUBQ10 for integrase target construction
195887L0-T4 tRBSC_mScarletLevel 0 tRBSC-mScarlet for integrase target construction
195888L0-I38 BpiI-pUBQ10-BpiILevel 0 pUBQ10 promoter between BpiI sites for target construction
195889L0-T6 Empty target Bxb1Level 0 Empty Bxb1 target: attP_Bxb1-BpiI-attB_Bxb1 with golden gate linker in pBLUNT plasmid.
195890L0-T1 Target PhiC31Level 0 PhiC31 target: attP_PhiC31-pUBQ10-attB_PhiC31 with golden gate linker in pBLUNT plasmid.
195891L0-I2 pPP2AA3-mutated GG0-GG1Level 0 pPP2AA3 promoter with GG0 and GG1 golden gate linkers
195892L0-I9 PhiC31 GG1-GG4Level 0 PhiC31 integrase with GG1 and GG4 golden gate linkers
195893L0-I10 PhiC31 GG2-GG4Level 0 PhiC31 integrase with GG2 GG4 golden gate linkers
195894L0-I11 PhiC31-NLS GG1-GG4Level 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkers
195895L0-I12 PhiC31-NLS GG2-GG4Level 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkers
195896L0-I14 DST GG4-GG5Level 0 DST (destabilization RNA tag: SAUR tag) with GG4 GG5 golden gate linkers
195897L0-I15 tUBQ1 GG4-GG6Level 0 tUBQ1 terminator with GG4 GG6 golden gate linkers
195898L0-I16 tUBQ1 GG5-GG6Level 0 tUBQ1 terminator with GG5 GG6 golden gate linkers
195899L0-I18 Ub-Arg GG1-GG2Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkers
195901L0-I23 Bxb1 GG1-GG4Level 0 Bxb1 integrase with GG1 and GG4 golden gate linkers
195902L0-I26 pUBQ10 GG0-GG1Level 0 pUBQ10 promoter with GG0 and GG1 golden gate linkers
195903L0-I32 pLBD16 GG0-GG1Level 0 pLBD16 promoter with GG0 and GG1 golden gate linkers
195904L0-I35 p35S-XVE-pEstradiol GG0-GG1Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)
195905L0-I39 PhiC31-Intein GG1-GG4Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers
195906L0-I40 Intein-PhiC31 GG1-GG4Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers
195907L0-I42 Tp901 GG1-GG4Level 0 Tp901 integrase with GG1 and GG4 golden gate linkers
195908pNL2080 pGATA23 GG0-GG1Level 0 pGATA23 promoter with GG0 and GG1 golden gate linkers
195909pNL2114 pARF19 GG0-GG1Level 0 pARF19 promoter with GG0 and GG1 golden gate linkers
195910pNL2119 pARF7 GG0-GG1Level 0 pARF7 promoter with GG0 and GG1 golden gate linkers
195911L1_ARF7_1Level 1 PhiC31 cassette with LR dvp promoter: pARF7-PhiC31-tUBQ1
195912L1_GATA23_2Level 1 PhiC31 cassette with LR dvp promoter: pGATA23-PhiC31-NLS-tUBQ1
195913L1_GATA23_1Level 1 PhiC31 cassette with LR dvp promoter: pGATA23-PhiC31-tUBQ1
195914L1_ARF19_2Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-NLS-tUBQ1
195915L1_ARF19_1Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-tUBQ1
195916L1_ARF19_4Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-DST-tUBQ1
195917L1P1Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-tUBQ1
195918L1P2Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-tUBQ1
195919L1P4Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-DST-tUBQ1
195920L1P5Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-DST-tUBQ1
195921L1P16Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-tUBQ1
195922L1P17Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-tUBQ1
195923L1P18Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-DST-tUBQ1
195924L1P19Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-DST-tUBQ1
195925L1P22Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-tUBQ1
195926L1P23Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-tUBQ1
195927L1P24Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-DST-tUBQ1
195928L1P25Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-DST-tUBQ1
195929L1P27Level 1 PhiC31 cassette with constitutive promoter: p35S-UbArg-PhiC31NLS-tUBQ1
195930L1P32Level 1 PhiC31 cassette with LR dvp promoter: pLBD16-PhiC31-tUBQ1
195931L1P33Level 1 PhiC31 cassette with LR dvp promoter : pLBD16-PhiC31NLS-tUBQ1
195932L1P38Level 1 PhiC31 cassette with estradiol inducible promoter: p35S_XVE-PhiC31-tUBQ1
195933L1P46Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3:PhiC31:t35S
195934L1P47Level 1 PhiC31 cassette with constitutive promoter : pPP2AA3:PhiC31-NLS:t35S
195935L1P56Level 1 PhiC31 cassette with constitutive promoter : p35S:UbGln:PhiC31-NLS:tUBQ1
195936L1P59Level 1 PhiC31-intein(DnaE-Npu) cassette : p35S-PI-t35S
195937L1P60Level 1 Intein(DnaE-Ssp)-PhiC31 cassette pUBQ10-IP-tUBQ1
195938L1P63PhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35S
195939L1P78Level 1 pARF19 reporter : pARF19-mScarlet-NLS-tRBCS
195940L1P79Level 1 pGATA23 reporter : pGATA23-mScarlet-NLS-tRBCS
195941L1P80Level 1 pLBD16 reporter : pLBD16-mScarlet-NLS-tRBCS
195942L1P96Level 1 PhiC31 cassette with LR dvp promoter : pLBD16-PhiC31-DST-tUBQ1
195943L1P97Level 1 PhiC31 cassette with LR dvp promoter : pGATA23-PhiC31-DST-tUBQ1
195944L1B1Level 1 Bxb1 cassette with constitutive promoter : pPP2AA3-Bxb1-tUBQ1
195945T1P/pUBQ10(mTurq->mScarlet)PhiC31 target with pUBQ10 switching from mTurq to mScarlet
195946T1B/pUBQ10(mTurq->mScarlet)Bxb1 target with pUBQ10 switching from mTurq to mScarlet
195947T1T/pUBQ10(mTurq->mScarlet)Tp901 target with pUBQ10 switching from mTurq to mScarlet

Antibodies from Article