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Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection.

Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM
Cell Rep. 2022 Dec 13;41(11):111817. doi: 10.1016/j.celrep.2022.111817. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
188698p5e irg1Gateway promoter element for the zebrafish irg1 gene
188699pME VIVIT NSGateway middle element encoding the VIVIT peptide (no stop codon)
188703pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBILentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs
188704pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNALentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2
188705pLV hUbC-Cas9-P2A-Puro_ST-sgRNALentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci
188706pLV VEET-GFPLentivirus transfer plasmid encoding VEET-GFP
188707pLV VIVIT-GFPLentivirus transfer plasmid encoding VIVIT-GFP
188708phU6 NFATC2sgRNA plasmid encoding hU6 promoter driving an anti-NFATC2 sgRNA
188709pmU6 NFATC2sgRNA plasmid encoding mU6 promoter driving an anti-NFATC2 sgRNA
188710p7SK NFATC2sgRNA plasmid encoding 7SK promoter driving an anti-NFATC2 sgRNA
188711phH1 NFATC2sgRNA plasmid encoding hH1 promoter driving an anti-NFATC2 sgRNA
188712phU6 STsgRNA plasmid encoding hU6 promoter driving an anti-safe targeting sgRNA
188713pmU6 STsgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNA
188714p7SK STsgRNA plasmid encoding 7SK promoter driving an anti-safe targeting sgRNA
188715phH1 STsgRNA plasmid encoding hH1 promoter driving an anti-safe targeting sgRNA

Antibodies from Article