Protein-protein interactions in the Mla lipid transport system probed by computational structure prediction and deep mutational scanning.
MacRae MR, Puvanendran D, Haase MAB, Coudray N, Kolich L, Lam C, Baek M, Bhabha G, Ekiert DC
J Biol Chem. 2023 Apr 24:104744. doi: 10.1016/j.jbc.2023.104744.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195625 | pBEL2511 | Complementation of MlaA(WT) |
| 195626 | pBEL2512 | Complementation of MlaA(delta238-251) |
| 195627 | pBEL2513 | Complementation of MlaA(delta227-251) |
| 195628 | pBEL2639 | Complementation of MlaA(I241N) |
| 195629 | pBEL2640 | Complementation of MlaA(L245N) |
| 195630 | pBEL2641 | Complementation of MlaA(I248N) |
| 195631 | pBEL2644 | Complementation of MlaA(D198N) |
| 195632 | pBEL2663 | Complementation of MlaA(F223N) |
| 195633 | pBEL2664 | Complementation of MlaA(L230N) |
| 195634 | pBEL2665 | Complementation of MlaA(delta244-251) |
| 195635 | pBEL1092 | Expression/purification of 6xHis-TEV-sfGFP |
| 195636 | pBEL2707 | Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251) |
| 195637 | pBEL2753 | Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N) |
| 195638 | pBEL2754 | Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N) |
| 195639 | pBEL2755 | Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N) |
| 195640 | pBEL2756 | Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251) |
| 195641 | pBEL2757 | Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251) |
| 195642 | pBEL2403 | Complementation of MlaC(WT) |
| 195643 | pBEL2521 | Complementation of MlaC(A34R) |
| 195644 | pBEL2522 | Complementation of MlaC(A108R) |
| 195645 | pBEL2523 | Complementation of MlaC(A163R) |
| 195646 | pBEL2524 | Complementation of MlaC(A168R) |
| 195647 | pBEL2525 | Complementation of MlaC(D165I) |
| 195648 | pBEL2526 | Complementation of MlaC(E169R) |
| 195649 | pBEL2527 | Complementation of MlaC(G170K) |
| 195650 | pBEL2528 | Complementation of MlaC(I130D) |
| 195651 | pBEL2530 | Complementation of MlaC(I137D) |
| 195652 | pBEL2531 | Complementation of MlaC(L76D) |
| 195653 | pBEL2532 | Complementation of MlaC(N155A) |
| 195654 | pBEL2533 | Complementation of MlaC(N179E) |
| 195655 | pBEL2534 | Complementation of MlaC(Q115A) |
| 195656 | pBEL2535 | Complementation of MlaC(R134E) |
| 195657 | pBEL2536 | Complementation of MlaC(R147D) |
| 195658 | pBEL2537 | Complementation of MlaC(R153A) |
| 195659 | pBEL2538 | Complementation of MlaC(R186A) |
| 195660 | pBEL2539 | Complementation of MlaC(T116D) |
| 195661 | pBEL2540 | Complementation of MlaC(T176R) |
| 195662 | pBEL2541 | Complementation of MlaC(V68R) |
| 195663 | pBEL2542 | Complementation of MlaC(V135R) |
| 195664 | pBEL2543 | Complementation of MlaC(V146R) |
| 195665 | pBEL2544 | Complementation of MlaC(V171R) |
| 195666 | pBEL2545 | Complementation of MlaC(Y72A) |
| 195667 | pBEL2546 | Complementation of MlaC(Y82K) |
| 195668 | pBEL2547 | Complementation of MlaC(Y105A) |
| 195669 | pBEL2548 | Complementation of MlaC(Y164R) |
| 195670 | pBEL2552 | Expression/purification of 6xHis-TEV-MlaC(A168R) |
| 195671 | pBEL2553 | Expression/purification of 6xHis-TEV-MlaC(D165I) |
| 195672 | pBEL2554 | Expression/purification of 6xHis-TEV-MlaC(E169R) |
| 195673 | pBEL2555 | Expression/purification of 6xHis-TEV-MlaC(G170K) |
| 195674 | pBEL2556 | Expression/purification of 6xHis-TEV-MlaC(I130D) |
| 195675 | pBEL2559 | Expression/purification of 6xHis-TEV-MlaC(L76D) |
| 195676 | pBEL2560 | Expression/purification of 6xHis-TEV-MlaC(N155A) |
| 195677 | pBEL2561 | Expression/purification of 6xHis-TEV-MlaC(N179E) |
| 195678 | pBEL2563 | Expression/purification of 6xHis-TEV-MlaC(R134E) |
| 195679 | pBEL2564 | Expression/purification of 6xHis-TEV-MlaC(R147D) |
| 195680 | pBEL2565 | Expression/purification of 6xHis-TEV-MlaC(R153A) |
| 195681 | pBEL2566 | Expression/purification of 6xHis-TEV-MlaC(R186A) |
| 195682 | pBEL2568 | Expression/purification of 6xHis-TEV-MlaC(T176R) |
| 195683 | pBEL2572 | Expression/purification of 6xHis-TEV-MlaC(V171R) |
| 195684 | pBEL2573 | Expression/purification of 6xHis-TEV-MlaC(Y72A) |
| 195685 | pBEL2574 | Expression/purification of 6xHis-TEV-MlaC(Y82K) |
| 195686 | pBEL2576 | Expression/purification of 6xHis-TEV-MlaC(Y164R) |
| 195687 | pBEL1224 | Expression/purification of 6xHis-TEV-MlaDdelta153-183 |
| 195688 | pBEL1223 | Expression/purification of 6xHis-TEV-MlaDdelta173-183 |
| 195689 | pBEL1954 | Expression/purification of 6xHis-TEV-MlaD(F118A/L123A) |
| 195690 | pBEL1909 | Expression/purification of 6xHis-TEV-MlaD(F118A) |
| 195691 | pBEL1910 | Expression/purification of 6xHis-TEV-MlaD(L123A) |
| 195692 | pBEL1936 | Complementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB |
| 195693 | pBEL1935 | Complementation of MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB |
| 195694 | pBEL1953 | Complementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB |
| 195695 | pBEL1933 | Complementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaB |
| 195696 | pBEL1934 | Complementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaB |
| 195697 | pBEL2319 | Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC |
| 196024 | pBEL1203 | Expression/purification of 6xHis-TEV-MlaC |
| 196025 | pBEL1160 | Expression/purification of 6xHis-TEV-MlaD |
| 196026 | pBEL1161 | Expression/purification of 6xHis-TEV-MlaDdelta141-183 |
| 196027 | pBEL1195 | Complementation of MlaF-MlaE-MlaD-MlaC-MlaB |
| 196028 | pBEL2766 | Complementation of MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB |