GPI-anchored glutathione S-transferase as marker allows affinity sorting of transfection-positive cells.
Ma S, Yang L, Zuo Q, Huang Q
Front Mol Biosci. 2022 Sep 29;9:1016090. doi: 10.3389/fmolb.2022.1016090. eCollection 2022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213713 | pGST-EGFP-GPIDAF | Express the affinity sorting tag GST-EGFP- GPIDAF. |
| 213714 | pGST-EGFP-GPIBY55 | Express the affinity sorting tag GST-EGFP- GPIBY55. |
| 213715 | pGST-EGFP-GPICEAM7 | Express the affinity sorting tag GST-EGFP- GPICEAM7. |
| 213716 | pLKO.1-GST-EGFP-GPIDAF | For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an hPGK promoter. |
| 213717 | pLKO.1-GST-EGFP-GPIBY55 | For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 driven by an hPGK promoter. |
| 213718 | pLKO.1-GST-EGFP-GPICEAM7 | For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an hPGK promoter. |
| 213719 | psgRNA-GST-EGFP-GPIDAF | To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter. |
| 213720 | psgRNA-GST-EGFP-GPIBY55 | To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter. |
| 213721 | psgRNA-GST-EGFP-GPICEAM7 | To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter. |
| 213722 | pcDNA3.1-GST-EGFP-GPIDAF | For overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an SV40 promoter. |
| 213723 | pcDNA3.1-GST-EGFP-GPICEAM7 | For overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter. |