A Gene Transfer-Positive Cell Sorting System Utilizing Membrane-Anchoring Affinity Tag.
Yang L, Cui L, Ma S, Zuo Q, Huang Q
Front Bioeng Biotechnol. 2022 Jun 16;10:930966. doi: 10.3389/fbioe.2022.930966. eCollection 2022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213704 | pTST-EGFP-GPIDAF | Express the affinity sorting tag TST-EGFP- GPIDAF. |
| 213705 | pTST-EGFP-GPICEAM7 | Express the affinity sorting tag TST-EGFP- GPICEAM7. |
| 213706 | pTST-EGFP-GPIBY55 | Express the affinity sorting tag TST-EGFP- GPIBY55. |
| 213707 | pLKO.1-TST-EGFP-GPIBY55 | For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter. |
| 213708 | pLKO.1-TST-EGFP-GPIDAF | For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter. |
| 213709 | pCRISPR/eCas9-GPIBY55 | To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters. |
| 213710 | pCRISPR/eCas9-GPIDAF | To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters. |
| 213711 | pcDNA3.1-TST-EGFP-GPIBY55 | For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter. |
| 213712 | pcDNA3.1-TST-EGFP-GPIDAF | For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter. |