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Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types.

Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin JA, Schnitzer MJ, Pieribone VA
Science. 2022 Nov 4;378(6619):eabm8797. doi: 10.1126/science.abm8797. Epub 2022 Nov 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
195518pAAV-Syn-Ace-mNeon2Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter
195520pAAV-Syn-VARNAM2Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter
195521pAAV-Syn-pAceRRed fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter
195522pAAV-Syn-Ace-mNeon2-Kv2.1PRGreen fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted
195523pAAV-Syn-pAce-Kv2.1PRGreen fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted
195524pAAV-Syn-VARNAM2-Kv2.1PRRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted
195525pAAV-Syn-pAceR-Kv2.1PRRed fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted
195526pAAV-CaMKII-Ace-mNeon2Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter
195527pAAV-CaMKII-VARNAM2Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter
195528pAAV-CaMKII-Ace-mNeon2-Kv2.1PRGreen fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted
195529pAAV-CaMKII-pAce-Kv2.1PRGreen fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted
195530pAAV-CaMKII-VARNAM2-Kv2.1PRRed fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted
195531pAAV-CaMKII-pAceR-Kv2.1PRRed fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted
195532pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PRCre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targeted
195533pAAV-CAG-DIO-VARNAM2-Kv2.1PRCre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted
195534pAAV-CAG-DIO-pAceR-Kv2.1PRCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted
195535pAAV-CAG-fDIO-pAce-Kv2.1PRFlp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted
195536pAAV-CAG-fDIO-VARNAM2-Kv2.1PRFlp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

Antibodies from Article