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A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene editing in Drosophila.

Doll RM, Boutros M, Port F
Sci Adv. 2023 Sep;9(35):eadj1568. doi: 10.1126/sciadv.adj1568. Epub 2023 Aug 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
195514pAct-dCBEevoAPOBEC1Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila
195515pAct-dCBEevoCDA1Ubiquitous expression of dCBEevoCDA1 in Drosophila
205152pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

Antibodies from Article