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Tim Brown Lab: Bacterial expression plasmids of HaloTag v7 variants

Unpublished

Plasmids from Article

ID Plasmid Purpose
195486pRSET.HaloTag.v7.AviTagBacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.
195487pRSET.HaloTag.v7.CoOp.Cys-lessBacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

Antibodies from Article