Mutations in the B30.2 and the central helical scaffold domains of pyrin differentially affect inflammasome activation.
Chirita D, Bronnec P, Magnotti F, Dalmon S, Martin A, Popoff M, Gerfaud-Valentin M, Seve P, Belot A, Contis A, Duquesne A, Nocturne G, Lemelle I, Georgin-Lavialle S, Boursier G, Touitou I, Jamilloux Y, Henry T
Cell Death Dis. 2023 Mar 25;14(3):213. doi: 10.1038/s41419-023-05745-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195474 | pInducer 21-3xFlag-hMEFV-S580X | pInducer21 plasmid containing the human MEFV gene with the S580X mutation (stop codon leading to the truncation of the B30.2 domain of the pyrin protein) with a 3xFlag tag at the N-terminus |
| 195475 | pInducer21-3xFlag-hMEFV-Q426R | pInducer21 plasmid containing the human MEFV gene with the Q426R mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminus |
| 195477 | pInducer 21-3xFlag-hMEFV-F479L | pInducer21 plasmid containing the human MEFV gene with the F479L mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminus |
| 195478 | pInducer 21-3xFlag-hMEFV-ΔPYD | pInducer21 plasmid containing the human MEFV gene with a deletion of residues 1 - 92 (the resulting pyrin protein lacks the PYD domain) with a 3xFlag tag at the N-terminus |
| 195479 | pInducer 21-3xFlag-hMEFV-ΔPLD | pInducer21 plasmid containing the human MEFV gene with a deletion of residues 88 - 310 (the resulting pyrin protein lacks the Phosphorylated Linker Domain domain) with a 3xFlag tag at the N-terminus |