CRISPR-Cas12a base editors confer efficient multiplexed genome editing in rice.
Cheng Y, Zhang Y, Li G, Fang H, Sretenovic S, Fan A, Li J, Xu J, Que Q, Qi Y
Plant Commun. 2023 Apr 13:100601. doi: 10.1016/j.xplc.2023.100601.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195366 | pYPQ230-D156R (ttLbCas12a) | A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a |
| 195367 | dpYPQ230-D156R-CBE1 | dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG |
| 195368 | dpYPQ230-D156R-CBE2 | dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN |
| 195369 | dpYPQ230-D156R-CBE4 | dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6 |
| 195370 | dpYPQ230-D156R-ABE4 | dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6 |
| 195371 | dpYPQ230-D156R-ABE5 | dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8 |
| 195569 | dpYPQ230-D156R-ABE8 | dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3 |