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Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids.

Nguyen LT, Rananaware SR, Yang LG, Macaluso NC, Ocana-Ortiz JE, Meister KS, Pizzano BLM, Sandoval LSW, Hautamaki RC, Fang ZR, Joseph SM, Shoemaker GM, Carman DR, Chang L, Rakestraw NR, Zachary JF, Guerra S, Perez A, Jain PK
Cell Rep Med. 2023 May 16;4(5):101037. doi: 10.1016/j.xcrm.2023.101037. Epub 2023 May 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
1953396xHis-MBP-BrCas12b-RFNDExpresses Engineered BrCas12b in Bacteria
1953406xHis-MBP-BrCas12b-FNLDTAExpresses Engineered BrCas12b in Bacteria

Antibodies from Article