Raj Chari Lab: Various CRISPR/Cas9 backbones
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195294 | pGMC00009 (aka pRC0494) | Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP |
| 195295 | pGMC00010 (aka pRC0495) | Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP |
| 195304 | pGMC00020 (aka pRC0431) | Lentiviral expression vector derived from LEGO-G2 with blasticidin selection |
| 195305 | pGMC00021 (aka pRC0432) | Lentiviral expression vector derived from LEGO-G2 with puromycin selection |
| 195309 | pGMC00023 (aka pRC0434) | Lentiviral expression vector derived from LEGO-G2 with G418/Neomycin selection |
| 195315 | pGMC00024 (aka pCW-Cas9n-Blast) | Dox inducible lentiviral Cas9 nickase |
| 195316 | pGMC00025 (aka pCW-dCas9-Blast) | Dox inducible lentiviral dead Cas9 |
| 195318 | pGMC00026 (aka pRC0462) | Lentiviral guide RNA backbone derived from pSB700 with neomycin/G418 |
| 195319 | pGMC00017 (aka pRC0162) | Lentiviral guide RNA backbone derived from pSB700 with puromycin |
| 195320 | pGMC00018 (aka pRC0082) | Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction |
| 195321 | pGMC00022 (aka pRC0433) | Lentiviral expression vector derived from LEGO-G2 with hygromycin selection |