Precise transcript targeting by CRISPR-Csm complexes.
Colognori D, Trinidad M, Doudna JA
Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195237 | pDAC439 | Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown) |
| 195238 | pDAC435 | Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown) |
| 195239 | pDAC446 | Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown) |
| 195240 | pDAC627 | Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown) |
| 195241 | pDAC569 | Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering) |
| 195242 | pDAC565 | Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging) |