The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner.
Garbett D, LaLonde DP, Bretscher A
J Cell Biol. 2010 Oct 18;191(2):397-413. doi: 10.1083/jcb.201004115. Epub 2010 Oct 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195207 | GFP-NHERF1 3xSA | Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A) |
| 195208 | GFP-NHERF1 3xSD | Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D) |
| 195209 | GFP-NHERF1 F355R | Mammalian expression vector for GFP tagged NHERF1. Ezrin interaction-deficient mutant |
| 195210 | GFP-NHERF1 PDZ1* | Mammalian expression vector for GFP tagged NHERF1. PDZ1 GYGF>GYAA motif mutant |
| 195211 | GFP-NHERF1 PDZ1/2* | Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant |
| 195212 | GFP-NHERF1 PDZ2* | Mammalian expression vector for GFP tagged NHERF1. PDZ2 GYGF>GYAA motif mutant |
| 195213 | GFP-NHERF1 WT | Mammalian expression vector for GFP tagged NHERF1. |