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Stephanie Roessler Lab: Chromosome 8p-engineering reveals increased metastasis targetable by patient-specific synthetic lethality

Unpublished

Plasmids from Article

ID Plasmid Purpose
195177pDEST26-MSRA-C-HAVector for transient expression of MSRA gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
195178pDEST26-NAT1-C-HAVector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
195179pDEST26-PPP2CB-C-HAVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
195181pTRIPZ-NUDT18-C-FLAG-PuroLentiviral vector for DOX-inducible NUDT18 gene expression with C-terminal FLAG tag. Gateway expression system. Selection by puromycin.
195182pTRIPZ-NUDT17-C-HA-PuroLentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.
195183pX458-tRNA-SpCas9Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

Antibodies from Article