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An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion.

Nguyen TT, Voeltz GK
Elife. 2022 Nov 30;11:e84279. doi: 10.7554/eLife.84279. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
195155V5-TurboID-Mfn1mammalian expression of TurboID tagged Mfn1
195156V5-TurboID-Mfn1E209Amammalian expression of TurboID tagged Mfn1E209A
195157ABHD16A-mNGmammalian expression of ABHD16A tagged with mNeonGreen
195158Stim1NTD-ABHD16Acyto-mNGmammalian expression of ER localized ABHD16A tagged with mNeonGreen
195159Tom70NTD-ABHD16Acyto-mNGmammalian expression of outer mitochondrial membrane localized ABHD16A tagged with mNeonGreen
195160siRNA-resistant ABHD16A-mNGmammalian expression of siRNA-resistant ABHD16A tagged with mNeonGreen
195161siRNA-resistant ABHD16A-mCherrymammalian expression of siRNA-resistant ABHD16A tagged with mCherry
195162mNG-Mfn1mammalian expression of Mfn1 tagged with mNeonGreen
195163siRNA-resistant ABHD16A-Halomammalian expression of siRNA-resistant ABHD16A tagged with Halo tag
195164siRNA-resistant ABHD16A-SNAPmammalian expression of siRNA-resistant ABHD16A tagged with SNAP tag
195165siRNA resistant ABHD16A-S176AS306A-mNGmammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen
195166siRNA-resistant ABHD16A-H189AD194A-mNGmammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen
195167siRNA-resistant ABHD16A-S355A-mNGmammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen
195168siRNA-resistant ABHD16A-H189AD194A-Halomammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag
195169siRNA-resistant ABHD16A-S355A-Halomammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag
195170siRNA-resistant mNG-ORP8mammalian expression of siRNA-resistant ORP8 tagged with mNeonGreen

Antibodies from Article