A cohesin traffic pattern genetically linked to gene regulation.
Valton AL, Venev SV, Mair B, Khokhar ES, Tong AHY, Usaj M, Chan K, Pai AA, Moffat J, Dekker J
Nat Struct Mol Biol. 2022 Dec 8. doi: 10.1038/s41594-022-00890-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195101 | 221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin | Vector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system. |
| 195102 | pEN396-pCAGGS-TIR1-V5-2A-HygroR | Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system. |
| 195103 | pX459-sgRNA1-CTCF-prom | Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion). |
| 195104 | pX459-sgRNA2-CTCF-prom | Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion). |
| 195105 | pX459-sgRNA3-CTCF-3p-UTR | Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion). |
| 195106 | pX459-sgRNA4-CTCF-3p-UTR | Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion). |
| 195107 | 221-RPB1-AID-eGFP-2A-blasticidin | Targeting vector to introduce AID-eGFP tags in C-terminal of the human RPB1 gene. Blasticidin selection (2A-fusion). Auxin-inducible degron system. |
| 195108 | 221-AID-RPB1 | Targeting vector to introduce AID tag in N-terminal of the human RPB1 gene. Auxin-inducible degron system. |
| 195109 | pX459-sgRNA2-2-RPB1-C-term | Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion). |
| 195110 | pX459-sgRNA1-RPB1-N-term | Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion). |
| 195111 | pX459-sgRNA2-RPB1-N-term | Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion). |
| 195112 | pX459-sgRNA3-RPB1-N-term | Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion). |