Creb5 establishes the competence for Prg4 expression in articular cartilage.

Zhang CH, Gao Y, Jadhav U, Hung HH, Holton KM, Grodzinsky AJ, Shivdasani RA, Lassar AB
Commun Biol. 2021 Mar 12;4(1):332. doi: 10.1038/s42003-021-01857-0. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
195037	pGL4.10 E4E3E2E1-Prg4 promoter-luciferase	Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
195036	pGL4.10 E3E2E1-Prg4 promoter-luciferase	Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
195035	GL4.10 E2E1- Prg4 promoter-luciferase	Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
195034	GL4.10 Prg4 promoter-luciferase	Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
195033	GAL4-Creb5 (1- 128) C18/C23 S	GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)
195032	GAL4-Creb5 (1- 128) T59/T61 A	GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)
195031	GAL4-Creb5 (1- 128)	GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)
195030	GAL4-Creb5 (1- 508)	GAL4-Creb5 fusion construct (full length, 508aa)
195029	GAL4(1-147)	The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector
195028	pInducer20-iCreb5(T59/T61D)(HA)	HA-tagged Creb5(T59/T61D) in pInducer20 lentivirus destination vector