Characterization of Integrase and Excisionase Activity in a Cell-Free Protein Expression System Using a Modeling and Analysis Pipeline.

Pandey A, Rodriguez ML, Poole W, Murray RM
ACS Synth Biol. 2023 Feb 17;12(2):511-523. doi: 10.1021/acssynbio.2c00534. Epub 2023 Jan 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
195374	pTet-Bxb1-CFP	Expresses Bxb1 integrase fused with CFP for fluorescence
195570	pTet-Bxb1-Xis-mScarlet	Expresses Bxb1 excisionase fused with mScarlet for fluorescence
195571	attB-P7-attP-YFP	A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.