A molecular switch for the orientation of epithelial cell polarization.
Bryant DM, Roignot J, Datta A, Overeem AW, Kim M, Yu W, Peng X, Eastburn DJ, Ewald AJ, Werb Z, Mostov KE
Dev Cell. 2014 Oct 27;31(2):171-87. doi: 10.1016/j.devcel.2014.08.027. Epub 2014 Oct 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195220 | GFP-PODXL FBM* | Mammalian expression vector containing GFP tagged Podocalyxin. With H477/R479/S481>AAA mutation. |
| 195221 | GFP-PODXL FBM*/PBM* | Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL. |
| 195223 | GFP-PODXL S481A | Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant. |
| 195224 | GFP-PODXL S481D | Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphomimetic mutant. |
| 195225 | pLKO-cfNHERF1_4 | shRNA to canine NHERF1. |
| 195226 | pLKO-cfNHERF1_5 | shRNA to canine NHERF1. |
| 195227 | pLKO-cfNHERF2_4 | shRNA to canine NHERF2. |
| 195228 | pLKO-cfNHERF2_5 | shRNA to canine NHERF2. |
| 195229 | 3XHA-PP2A-Ba | Retroviral vector containing HA tagged PP2A |
| 195230 | mVenus-PKCB1 K371R | Retroviral vector containing GFP tagged PKCB1. Kinase inactive mutant. |
| 195232 | GFP-PKCBII | Mammalian expression vector containing GFP tagged PKCBII. |