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GFP fusions of Sec-routed extracellular proteins in Staphylococcus aureus reveal surface-associated coagulase in biofilms.

Evans DCS, Khamas AB, Marcussen L, Rasmussen KS, Klitgaard JK, Kallipolitis BH, Nielsen J, Otzen DE, Leake MC, Meyer RL
Microb Cell. 2023 Jun 28;10(7):145-156. doi: 10.15698/mic2023.07.800. eCollection 2023 Jul 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
194913pRMC2-msfGFPTetracycline inducible expression of msfGFP in Staphylococci
194914pRMC2-sec:msfGFPTetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
194915pRMC2-tat:msfGFPTetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

Antibodies from Article