Recording physiological history of cells with chemical labeling.
Huppertz MC, Wilhelm J, Grenier V, Schneider MW, Falt T, Porzberg N, Hausmann D, Hoffmann DC, Hai L, Tarnawski M, Pino G, Slanchev K, Kolb I, Acuna C, Fenk LM, Baier H, Hiblot J, Johnsson K
Science. 2024 Feb 23;383(6685):890-897. doi: 10.1126/science.adg0812. Epub 2024 Feb 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194659 | pET-51b(+)-cpHalo∆ | Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria |
| 194660 | pET-51b(+)-cpHaloTag141/145 | Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria |
| 194661 | pET-51b(+)-cpHaloTag154/156 | Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria |
| 194662 | pET-51b(+)-Caprola_01 | Protein production of the split HaloTag based calcium recorder Caprola_01 in bacteria |
| 194663 | pET-51b(+)-Caprola_02 | Protein production of the split HaloTag based calcium recorder Caprola_02 in bacteria |
| 194664 | pET-51b(+)-Caprola_03 | Protein production of the split HaloTag based calcium recorder Caprola_03 in bacteria |
| 194665 | pET-51b(+)-Caprola_04 | Protein production of the split HaloTag based calcium recorder Caprola_04 in bacteria |
| 194666 | pET-51b(+)-Caprola_05 | Protein production of the split HaloTag based calcium recorder Caprola_05 in bacteria |
| 194667 | pET-51b(+)-Caprola_06 | Protein production of the split HaloTag based calcium recorder Caprola_06 in bacteria |
| 194668 | pET-51b(+)-Caprola_07 | Protein production of the split HaloTag based calcium recorder Caprola_07 in bacteria |
| 194669 | pET-51b(+)-Caprola_08 | Protein production of the split HaloTag based calcium recorder Caprola_08 in bacteria |
| 194670 | pET-51b(+)-Caprola_09 | Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria |
| 194671 | pET-51b(+)-Caprola_10 | Protein production of the split HaloTag based calcium recorder Caprola_10 in bacteria |
| 194672 | pET-51b(+)-Caprola_11 | Protein production of the split HaloTag based calcium recorder Caprola_11 in bacteria |
| 194673 | pET-51b(+)-Caprola_12 | Protein production of the split HaloTag based calcium recorder Caprola_12 in bacteria |
| 194674 | pET-51b(+)-Caprola_13 | Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria |
| 194675 | pET-51b(+)-Caprola_14 | Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria |
| 194676 | pET-51b(+)-Caprola_15 | Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria |
| 194678 | pcDNA3.1-CHRM3-cpHalo∆-mEGFP | CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP |
| 194679 | pcDNA3.1-DRD2-cpHalo∆-mEGFP | CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP |
| 194681 | pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP | CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP |
| 194682 | pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP | CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP |
| 194683 | pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP | CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP |
| 194684 | pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP | CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP |
| 194685 | pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP | CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP |
| 194686 | pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP | CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP |
| 194687 | pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP | CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP |
| 194688 | pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40 | hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction |
| 194689 | pAAV2_hSyn_NES-Caprola_05-mEGFP_WRPE-SV40 | hSyn1 driven expression of the calcium recorder Caprola_05 fused to mEGFP for neuronal expression through AAV transduction |
| 194690 | pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40 | hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction |
| 194691 | pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40 | hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction |
| 194692 | pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40 | hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction |
| 194693 | pLKO.1-puro_CMV_NES-Caprola_06-mEGFP | CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction |
| 194694 | pLKO.1-puro_CMV_NES-Caprola_on-mEGFP | CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction |
| 194695 | pLKO.1-puro_CMV_NES-Caprola_off-mEGFP | CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction |
| 194696 | pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP | HuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafish |
| 194697 | pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP | HuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafish |
| 194698 | pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP | HuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafish |
| 205703 | pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett | CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines |
| 205704 | pJFRC7-20XUAS-IVS-NES_Caprola5-mEGFP | Gal4 driven expression of EGFP-Caprola5 for targeted expression in Drosophila flies |