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Intracellular phase separation of globular proteins facilitated by short cationic peptides.

Yeong V, Wang JW, Horn JM, Obermeyer AC
Nat Commun. 2022 Dec 22;13(1):7882. doi: 10.1038/s41467-022-35529-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
194616pVY-1 sfGFP-tag1fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194617pVY-2 sfGFP-tag2fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194618pVY-3 sfGFP-tag3fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194619pVY-4 GFP(0)-tag1fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194620pVY-5 GFP(0)-tag2fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194621pVY-6 GFP(0)-tag3fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194622pVY-7 GFP(+6)-tag1fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194623pVY-8 GFP(+6)-tag2fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194624pVY-9 GFP(+6)-tag3fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194625pVY-10 mScarletI-tag2fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194626pVY-11 mScarletI-tag3fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli
194627pVY-12 CAT-tag3antibiotic resistance enzyme with cationic peptide tag to promote biomolecular condensate formation in E. coli
194628pVY-14 TC-CATantibiotic resistance enzyme with tetracysteine tag for visualization of enzyme
194629pVY-15 TC-CAT-tag3antibiotic resistance enzyme with cationic peptide tag and tetracysteine tag
194630pVY-16 TC-TEM1antibiotic resistance enzyme with tetracysteine tag for visualization of enzyme
194631pVY-17 TC-TEM1-tag3antibiotic resistance enzyme with cationic peptide tag and tetracysteine tag
194632pVY-18 TC-sfGFPfluorescent protein with tetracysteine tag
194633pVY-19 TC-sfGFP-tag3fluorescent protein with cationic peptide tag and tetracysteine tag

Antibodies from Article