Formation of Biomolecular Condensates in Bacteria by Tuning Protein Electrostatics.
Yeong V, Werth EG, Brown LM, Obermeyer AC
ACS Cent Sci. 2020 Dec 23;6(12):2301-2310. doi: 10.1021/acscentsci.0c01146. Epub 2020 Nov 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199160 | pET-6xHis-GFP(+6) | used to express a supercharged GFP variant in E. coli |
| 199161 | pET-6xHis-GFP(+12) | used to express a supercharged GFP variant in E. coli |
| 199162 | pET-6xHis-GFP(+18) | used to express a supercharged GFP variant in E. coli |
| 199163 | pET-6xHis-GFP(+24) | used to express a supercharged GFP variant in E. coli |
| 199164 | pET-6xHis-GFP(+30) | used to express a supercharged GFP variant in E. coli |
| 199165 | pET-6xHis-GFP(+36) | used to express a supercharged GFP variant in E. coli |
| 199166 | pET-6xHis-GFP(0) | used to express a neutral GFP variant in E. coli |
| 199167 | pET-6xHis-GFP(-30) | used to express a supercharged GFP variant in E. coli |
| 199168 | pET-6xHis-GFP(+36)-DL4 | used to express a supercharged GFP variant that forms inclusion bodies in E. coli |
| 199171 | pET-6xHis-sfGFP | used to express a superfolder GFP in E. coli for comparison to supercharged variants |