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Auxotrophy-Independent Plasmid Shuttle Vectors for Applications in Diverse Yeasts

Smith JR, Sislak CD, Fernandez Mendoza P, Carmichael L, Lewis AG, Chen A, Jiang GZ, Gibney PA
Appl Microbiol. 2024; 4(1):453-469. doi: 10.3390/applmicrobiol4010031 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
194522pRSII41KLow copy vector backbone with G418 selectivity, contains MCS
194523pRSII42KHigh copy vector backbone with G418 selectivity, contains MCS
194524pRSII41NLow copy vector backbone with nourseothricin selectivity, contains MCS
194525pRSII42NHigh copy vector backbone with nourseothricin selectivity, contains MCS
194526pRSII41HLow copy vector backbone with hygromycin B selectivity, contains MCS
194527pRSII42HHigh copy vector backbone with hygromycin B selectivity, contains MCS
194528pRSII41K-TDH3prLow copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)
194529pRSII42K-TDH3prHigh copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)
194530pRSII41N-TDH3prLow copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)
194531pRSII42N-TDH3prHigh copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)
194532pRSII41H-TDH3prLow copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)
194533pRSII42H-TDH3prHigh copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)
194534pRSII41K-TDH3pr-mNeonLow copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon
194535pRSII42K-TDH3pr-mNeonHigh copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon
194536pRSII41N-TDH3pr-mNeonLow copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon
194537pRSII42N-TDH3pr-mNeonHigh copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon
194538pRSII41H-TDH3pr-mNeonLow copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon
194539pRSII42H-TDH3pr-mNeonHigh copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Antibodies from Article