CRISPR FISHer enables high-sensitivity imaging of nonrepetitive DNA in living cells through phase separation-mediated signal amplification.
Lyu XY, Deng Y, Huang XY, Li ZZ, Fang GQ, Yang D, Wang FL, Kang W, Shen EZ, Song CQ
Cell Res. 2022 Nov;32(11):969-981. doi: 10.1038/s41422-022-00712-z. Epub 2022 Sep 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194503 | pHAGE-EFS-foldon-GFP- PCP | Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA |
| 194504 | pPUR-mU6-sgRNA-2XPP7 | Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system |