Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors.
Silva G, Rodrigues AF, Ferreira S, Matos C, Eleuterio RP, Marques G, Kucheryava K, Lemos AR, Sousa PMF, Castro R, Barbas A, Simao D, Alves PM
Biomolecules. 2023 Mar 2;13(3):459. doi: 10.3390/biom13030459.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194457 | pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G | CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring. |
| 194458 | pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G | CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring. |
| 194459 | pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G | CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring. |
| 194460 | pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G | CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring. |
| 194461 | pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G | CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring. |
| 194462 | pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G | CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring. |
| 194463 | pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G | CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring. |
| 194464 | pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G | CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring. |