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A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms.

Bogaert A, Van de Steene T, Vuylsteke M, Eyckerman S, Gevaert K
Methods Enzymol. 2023;684:253-287. doi: 10.1016/bs.mie.2023.02.003. Epub 2023 Mar 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
194453pMET7-EGFP-FKBP12Mammalian expression vector that expresses the EGFP protein with FKBP12 fused to it
194454pMET7-EDHFR-FKBP12Mammalian expression vector that expresses the EDHFR protein with FKBP12 fused to it
194455pMET7-GAG-FRBMammalian expression vector that expresses the HIV-1 GAG protein with FRB fused to it

Antibodies from Article