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ScEnSor Kit for Saccharomyces cerevisiae Engineering and Biosensor-Driven Investigation of the Intracellular Environment.

Torello Pianale L, Olsson L
ACS Synth Biol. 2023 Aug 8. doi: 10.1021/acssynbio.3c00124. (Link opens in a new window) PubMed (Link opens in a new window) Article


A subset of these plasmids is included in the Olsson - ScEnSor Kit.

Plasmids from Article

ID Plasmid Purpose
194403LT1_30_backbone_X2_integrationMulti Backbone with X2 homology arms for Saccharomyces cerevisiae
194404LT1_31_backbone_HO_integrationMulti Backbone with HO homology arms for Saccharomyces cerevisiae
194405LT1_63_TU1of2-6_X2homologyTU1 backbone (for TU #1 for plasmid with 2-6 TUs) with X2 homology arms for Saccharomyces cerevisiae
194406LT1_64_TU2of2_X2homologyTU2 backbone (for TU #2 for plasmid with 2 TUs) with X2 homology arms for Saccharomyces cerevisiae
194407LT1_65_TU2of3-6_X2homologyTU2 backbone (for TU #2 for plasmid with 3-6 TUs) with X2 homology arms for Saccharomyces cerevisiae
194408LT1_66_TU3of3_X2homologyTU3 backbone (for TU #3 for plasmid with 3 TUs) with X2 homology arms for Saccharomyces cerevisiae
194409LT1_67_TU3of4-6_X2homologyTU3 backbone (for TU #3 for plasmid with 4-6 TUs) with X2 homology arms for Saccharomyces cerevisiae
194410LT1_68_TU4of4_X2homologyTU4 backbone (for TU #4 for plasmid with 4 TUs) with X2 homology arms for Saccharomyces cerevisiae
194411LT1_69_TU4of5-6_X2homologyTU4 backbone (for TU #4 for plasmid with 5-6 TUs) with X2 homology arms for Saccharomyces cerevisiae
194412LT1_70_TU5of5_X2homologyTU5 backbone (for TU #5 for plasmid with 5 TUs) with X2 homology arms for Saccharomyces cerevisiae
194413LT1_71_TU5of6_X2homologyTU5 backbone (for TU #5 for plasmid with 6 TUs) with X2 homology arms for Saccharomyces cerevisiae
194414LT1_72_TU6of6_X2homologyTU6 backbone (for TU #6 for plasmid with 6 TUs) with X2 homology arms for Saccharomyces cerevisiae
194415LT1_75_Donor_Tag_RPL13A-mTurq2Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro
194416LT1_77_TU1of2-6_HOhomologyTU1 backbone (for TU #1 for plasmid with 2-6 TUs) with HO homology arms for Saccharomyces cerevisiae
194417LT1_78_TU2of2_HOhomologyTU2 backbone (for TU #2 for plasmid with 2 TUs) with HO homology arms for Saccharomyces cerevisiae
194418LT1_79_TU2of3-6_HOhomologyTU2 backbone (for TU #2 for plasmid with 3-6 TUs) with HO homology arms for Saccharomyces cerevisiae
194419LT1_80_TU3of3_HOhomologyTU3 backbone (for TU #3 for plasmid with 3 TUs) with HO homology arms for Saccharomyces cerevisiae
194420LT1_81_TU3of4-6_HOhomologyTU3 backbone (for TU #3 for plasmid with 4-6 TUs) with HO homology arms for Saccharomyces cerevisiae
194421LT1_82_TU4of4_HOhomologyTU4 backbone (for TU #4 for plasmid with 4 TUs) with HO homology arms for Saccharomyces cerevisiae
194422LT1_83_TU4of5-6_HOhomologyTU4 backbone (for TU #4 for plasmid with 5-6 TUs) with HO homology arms for Saccharomyces cerevisiae
194423LT1_84_TU5of5_HOhomologyTU5 backbone (for TU #5 for plasmid with 5 TUs) with HO homology arms for Saccharomyces cerevisiae
194424LT1_85_TU5of6_HOhomologyTU5 backbone (for TU #5 for plasmid with 6 TUs) with HO homology arms for Saccharomyces cerevisiae
194425LT1_86_TU6of6_HOhomologyTU6 backbone (for TU #6 for plasmid with 6 TUs) with HO homology arms for Saccharomyces cerevisiae
194426YN2_1_IL50_HOlocusExpresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae
194427LT2_15_UPRpro_X2sitePlasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein Response
194428LT2_32_PyruEth_X2sitePlasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)
194429LT2_33_PyruPro_X2sitePlasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)
194430LT2_34_EthPro_X2sitePlasmid for genome integration in X2 site expressing ResPro (Ethanol consumption Probe)

Antibodies from Article