Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via Systematic Analysis of Signal Peptide and beta-arrestin Binding Motif Variants.
Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194358 | pcDNA3_DRD1-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194359 | pcDNA3_SP-DRD1-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194360 | pcDNA3_DRD1-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194361 | pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194362 | pcDNA3_DRD2-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194363 | pcDNA3_SP-DRD2-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194364 | pcDNA3_DRD2-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194365 | pcDNA3_SP-DRD2-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194366 | pcDNA3_HTR2A-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194367 | pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194368 | pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194369 | pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194370 | pcDNA3_AVPR2-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194371 | pcDNA3_SP-AVPR2-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194372 | pcDNA3_AVPR2-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194373 | pcDNA3_SP-AVPR2-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194374 | pcDNA3_GCGR-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194375 | pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194376 | pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194377 | pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194378 | pcDNA3_GLP1R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194379 | pcDNA3_SP-GLP1R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194380 | pcDNA3_GLP1R-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194381 | pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA | Split TEV assays |
| 194382 | pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHA | Split TEV assays |
| 194383 | pGL4_10xUAS-MLPmin-luc2 | Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter |
| 194384 | pGL4_CRE-CMVmin-luc2 | Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter |
| 194385 | pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST | Gateway Destination vector for split TEV assays |
| 194386 | pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DEST | Gateway Destination vector for GPCR split TEV assays with V2R tail |