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Combining long-term circuit mapping and network transcriptomics with SiR-N2c.

Lee H, Ciabatti E, Gonzalez-Rueda A, Williams E, Nugent F, Mookerjee S, Morgese F, Tripodi M
Nat Methods. 2023 Apr;20(4):580-589. doi: 10.1038/s41592-023-01787-1. Epub 2023 Mar 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
194352pLenti-CMV-GFP-2A-G(N2c)-WPRELentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c strain
194353pLenti-CMV-CAG-GFP-EnvA(N2c)Lentivirus expressing eGFP and (EnvA-N2c Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies of CVS-N2c strain
194354pAAV-hSyn1-TVAmCherry-2A-G(N2c)AAV expressing TVA-mCherry fusion and Rabies Glycoprotein (G) of CVS-N2c strain under neuronal-specific promoter
194355pAAV-CMV-H2B-3xFLAG-2A-G(N2c)AAV constitutively expressing nuclear FLAG and Rabies Glycoprotein (G) of CVS-N2c strain

Antibodies from Article