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Generating minimum set of gRNA to cover multiple targets in multiple genomes with MINORg.

Lee RR, Cher WY, Wang J, Chen Y, Chae E
Nucleic Acids Res. 2023 Mar 15:gkad142. doi: 10.1093/nar/gkad142. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
194343pECNUS_P1sgRNA position entry clone 1
194344pECNUS_P2sgRNA position entry clone 2
194345pECNUS_P3sgRNA position entry clone 3
194346pECNUS_P4sgRNA position entry clone 4
194347pECNUS_P5sgRNA position entry clone 5
194348pECNUS_P6sgRNA position entry clone 6
194349pECNUS_P7sgRNA position entry clone 7
194350pECNUS_MP01CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter
194351pECNUS_MP02CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Antibodies from Article