Excitatory amino acid transporter 1 supports adult hippocampal neural stem cell self-renewal.
Rieskamp JD, Rosado-Burgos I, Christofi JE, Ansar E, Einstein D, Walters AE, Valentini V, Bruno JP, Kirby ED
iScience. 2023 Jun 8;26(7):107068. doi: 10.1016/j.isci.2023.107068. eCollection 2023 Jul 21.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
194281 | EF1a-dCas9-KRAB-GFP backbone | Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA |
194283 | U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP | EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA |
194284 | U6-NT sgRNA; EF1a-dCas9-KRAB-GFP | EF1a-dCas9-KRAB-GFP with nontarget sgRNA |