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Construction of a Set of Novel Transposon Vectors for Efficient Silencing of Protein and lncRNA Genes via CRISPR Interference.

Czarnek M, Kochan J, Wawro M, Myrczek R, Bereta J
Mol Biotechnol. 2023 Jan 28. doi: 10.1007/s12033-023-00675-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196074pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI(Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
196075pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.
196076pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI(Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
196077pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.
196078pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI(Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
196079pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.
196080pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI(Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
196081pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.
196082pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI(Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
196083pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.
196084pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI(Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
196085pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.
196086pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORADInducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.

Antibodies from Article