A synergy between site-specific and transient interactions drives the phase separation of a disordered, low-complexity domain.
Mohanty P, Shenoy J, Rizuan A, Mercado-Ortiz JF, Fawzi NL, Mittal J
Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2305625120. doi: 10.1073/pnas.2305625120. Epub 2023 Aug 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194263 | TDP-43 _CTD_5M→A_CR | Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CR |
| 194264 | TDP-43 _CTD_5M→A_IDR | Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to Alanine |
| 194265 | TDP-43 _CTD_5M→L_IDR | Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucine |
| 194266 | TDP-43 _CTD_5M→Y_IDR | Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosine |
| 194267 | TDP-43 _CTD_5A→M_IDR | Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionine |
| 194268 | TDP-43 _CTD_5A→M_IDR + 5M→A_CR | Expresses 6xHis-tagged C-terminal domain of TDP-43 with TDP-43 _CTD_5A→M_IDR + 5M→A_CR |
| 194269 | TDP-43 _CTD_6F→A | Expresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→A |