Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
bioRxiv 2021.04.20.440678
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194217 | pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407) | Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene |
| 194218 | PDGFR-TMD (MTK 3B) (pZYW025) | Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR |
| 194220 | MTK Lentivirus Single Cassette Destination Vector (pAN2414) | Backbone for constructing single casette expression plasmids using the Mammalian Tookit |
| 194221 | MTK Lentivirus Multi Cassette Destination Vector (pAN2424) | Backbone for constructing multi casette expression plasmids using the Mammalian Tookit |
| 194222 | SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031) | Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors |
| 194223 | T2A-mCherry (MTK 4a) (pAN3844) | Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry |
| 194224 | PGK Promoter (MTK 2) (pAN2822) | Mammalian Toolkit part 2 encoding the PGK promoter |
| 194225 | EF1a Promoter (MTK 2) (pAN2824) | Mammalian Toolkit part 2 containing the EF1a promoter |
| 194226 | PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037) | Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker |
| 194227 | LCB1_v1.3 (MTK 3a) (pZYW044) | Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1 |
| 194228 | LCB3_v1.2 (MTK 3a) (pZYW045) | Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3 |
| 194229 | PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046) | Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker |
| 194230 | PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047) | Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker |
| 194231 | EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048) | Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker |
| 194232 | EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049) | Lentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker |
| 194233 | SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077) | Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domain |
| 194234 | EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079) | Lentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker |
| 194235 | WPRE (MTK 4b) (pAN2410) | Mammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbones |