RNA recording in single bacterial cells using reprogrammed tracrRNAs.
Jiao C, Reckstadt C, Konig F, Homberger C, Yu J, Vogel J, Westermann AJ, Sharma CM, Beisel CL
Nat Biotechnol. 2023 Jan 5. doi: 10.1038/s41587-022-01604-8.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194209 | Sth1Cas9n cytosine base editor | Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording |
| 194210 | CJ8421_04975 Rptr2 and CJ8421_04975 mRNA | Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2 |
| 194211 | CJ8421_04975 Rptr2 recording plasmid | CJ8421_04975 Rptr2 targeted DNA sequence |
| 199718 | tracrRNA(scr)-CJ8421_04975 mRNA | Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence |