HIF1alpha-AS1 is a DNA:DNA:RNA triplex-forming lncRNA interacting with the HUSH complex.
Leisegang MS, Bains JK, Seredinski S, Oo JA, Krause NM, Kuo CC, Gunther S, Senturk Cetin N, Warwick T, Cao C, Boos F, Izquierdo Ponce J, Haydar S, Bednarz R, Valasarajan C, Fuhrmann DC, Preussner J, Looso M, Pullamsetti SS, Schulz MH, Jonker HRA, Richter C, Rezende F, Gilsbach R, Pfluger-Muller B, Wittig I, Grummt I, Ribarska T, Costa IG, Schwalbe H, Brandes RP
Nat Commun. 2022 Nov 2;13(1):6563. doi: 10.1038/s41467-022-34252-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194172 | pcDNA3.1+HIF1a-AS1 | Expresses lncRNA HIF1a-AS1 (spliced) |
| 194173 | pcDNA3.1+HIF1a-AS1_deltaExon2 | Expresses lncRNA HIF1a-AS1 Exon1 |
| 194174 | pcDNA3.1+HIF1a-AS1_deltaExon1 | Expresses lncRNA HIF1a-AS1 Exon2 |
| 194175 | pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78) | Expresses lncRNA HIF1a-AS1 mutant |
| 194176 | pcDNA3.1+HIF1a-AS1_1200_unspliced | Expresses first 1200 nt of lncRNA HIF1a-AS1 (unspliced) |
| 194178 | pCMV6-MPP8-10xHis | Expresses MPP8-10xHis tagged |
| 194179 | pCMV6-MPP8-W80A-10xHis | Expresses MPP8-W80A_10xHis |
| 194180 | pCMV6-MPP8-delta55-118-10xHis | Expresses MPP8-delta55-118_10xHis |
| 194181 | pCMV6-MPP8-delta2-441-10xHis | Expresses MPP8-delta2-441_10xHis |
| 194182 | pCMV6-MPP8-delta442-860-10xHis | Expresses MPP8-delta442-860_10xHis |
| 194183 | pCMV6-MPP8-delta600-728-10xHis | Expresses MPP8-delta600-728_10xHis |