Cryo-EM structures of Escherichia coli Ec86 retron complexes reveal architecture and defence mechanism.
Wang Y, Guan Z, Wang C, Nie Y, Chen Y, Qian Z, Cui Y, Xu H, Wang Q, Zhao F, Zhang D, Tao P, Sun M, Yin P, Jin S, Wu S, Zou T
Nat Microbiol. 2022 Sep;7(9):1480-1489. doi: 10.1038/s41564-022-01197-7. Epub 2022 Aug 18.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
194087 | pBB75-ncRNA | Expresses Ec86 ncRNA in Escherichia coli |
194088 | pET21b-rrt-His8 | Expresses Ec86 reverse transcriptase in Escherichia coli |
194089 | pET21b-effector-ncRNA-rrt-His8 | Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli |