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Cryo-EM structures of Escherichia coli Ec86 retron complexes reveal architecture and defence mechanism.

Wang Y, Guan Z, Wang C, Nie Y, Chen Y, Qian Z, Cui Y, Xu H, Wang Q, Zhao F, Zhang D, Tao P, Sun M, Yin P, Jin S, Wu S, Zou T
Nat Microbiol. 2022 Sep;7(9):1480-1489. doi: 10.1038/s41564-022-01197-7. Epub 2022 Aug 18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
194087pBB75-ncRNAExpresses Ec86 ncRNA in Escherichia coli
194088pET21b-rrt-His8Expresses Ec86 reverse transcriptase in Escherichia coli
194089pET21b-effector-ncRNA-rrt-His8Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli

Antibodies from Article