Targeting endogenous proteins for spatial and temporal knockdown using auxin-inducible degron in Caenorhabditis elegans.
Kurashina M, Mizumoto K
STAR Protoc. 2023 Jan 13;4(1):102028. doi: 10.1016/j.xpro.2022.102028.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194052 | pTK73.ChrV_oxTi365.gRNA | sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome |
| 194053 | Punc-4c.TIR1 | TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans |
| 194054 | AID-BFP-loxP_myo2_neoR | Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome |
| 194055 | AID-mTagBFP2-loxP_myo2_neoR-loxP | Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome |
| 194056 | ChrII_ttTi5605_loxP_myo2_neoR | Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome |
| 194057 | ChrV_oxTi365_loxP_myo2_neoR | Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome |
| 194058 | pTK73.ChrII_ttTi5605.sgRNA | sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome |