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Facile detection of mechanical forces across proteins in cells with STReTCh.

Zhong BL, Vachharajani VT, Dunn AR
Cell Rep Methods. 2022 Sep 2;2(9):100278. doi: 10.1016/j.crmeth.2022.100278. eCollection 2022 Sep 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
216532T7-LacO-Halo-STReTCh-RGDExpresses HaloTag-STReTCh-RGD fusion protein in bacteria
216710T7-LacO-Halo-RGD-STReTCh (Force Inert)Expresses HaloTag-RGD-STReTCh Force Inert Control in bacteria
216713pDEST14-Cys-SpyCatcher (Min)Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Antibodies from Article