Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase.
Tong H, Wang X, Liu Y, Liu N, Li Y, Luo J, Ma Q, Wu D, Li J, Xu C, Yang H
Nat Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01595-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193966 | AYBEv0.1 | A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter |
| 193967 | AYBEv3 | An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter |
| 193968 | pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA | Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter |