Automated, quantitative Fast FLIM-FRET (qF3): A step-by-step protocol to measure dissociation constants for protein-protein interactions in live-cell screening applications

Osterlund EJ, Hirmiz N, Pemberton JM, Fang Q, Andrews DW
Protocol Exchange (2022) (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
177405	Venus-7aa-ActA-pEGFP-C1	To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.