Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.
Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
195698 | pFSynW pHluorin-SYT9 IRES mRuby3 | Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter |
195699 | pFSynW Substance P-pHluorin IRES mRuby3 | Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter |
195700 | pFSynW Syt9 IRES mRuby3 | Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter |
195701 | pFSynW SYT9 D197, 199N IRES GFP | Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter |
195702 | pFSynW SYT9 D330, 332N IRES GFP | Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter |
195703 | pFSynW SYT9 D197, 199, 330, 332N IRES GFP | Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter |
195704 | pGEX SYT9 C2A D197,199N | Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations |
195705 | pGEX SYT9 C2B D330, 332N | Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations |
195706 | pGEX SYT9 C2AB D197,199,330,332N | Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations |
195707 | pGEX SYT9 C2A | Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain |
195708 | pGEX SYT9 C2B | Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain |
195709 | pGEX SYT9 C2AB | Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain |
195710 | pGEX SYT1 C2A | Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain |
195711 | pGEX SYT1 C2B | Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain |
195712 | pGEX SYT1 C2AB | Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain |
195713 | pET (SUMO)-Syt9 | Bacterial expression plasmid encoding SUMO-tagged full-length Syt9 |