Skip to main content
Addgene

Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.

Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
195698pFSynW pHluorin-SYT9 IRES mRuby3Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter
195699pFSynW Substance P-pHluorin IRES mRuby3Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter
195700pFSynW Syt9 IRES mRuby3Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter
195701pFSynW SYT9 D197, 199N IRES GFPLentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter
195702pFSynW SYT9 D330, 332N IRES GFPLentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter
195703pFSynW SYT9 D197, 199, 330, 332N IRES GFPLentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter
195704pGEX SYT9 C2A D197,199NBacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations
195705pGEX SYT9 C2B D330, 332NBacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations
195706pGEX SYT9 C2AB D197,199,330,332NBacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations
195707pGEX SYT9 C2ABacterial expression plasmid encoding GST-tagged SYT9 C2A domain
195708pGEX SYT9 C2BBacterial expression plasmid encoding GST-tagged SYT9 C2B domain
195709pGEX SYT9 C2ABBacterial expression plasmid encoding GST-tagged SYT9 C2AB domain
195710pGEX SYT1 C2ABacterial expression plasmid encoding GST-tagged SYT1 C2A domain
195711pGEX SYT1 C2BBacterial expression plasmid encoding GST-tagged SYT1 C2B domain
195712pGEX SYT1 C2ABBacterial expression plasmid encoding GST-tagged SYT1 C2AB domain
195713pET (SUMO)-Syt9Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

Antibodies from Article