Delivered complementation in planta (DCIP) enables measurement of peptide-mediated protein delivery efficiency in plants.
Wang JW, Squire HJ, Goh NS, Ni HM, Lien E, Wong C, Gonzalez-Grandio E, Landry MP
Commun Biol. 2023 Aug 12;6(1):840. doi: 10.1038/s42003-023-05191-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193860 | DCIP | Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium. |
| 193861 | cytoDCIP | Encodes mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium. |
| 193862 | 1BR9 | E. coli expression vector for N-ter GFP11 and C-ter R9 tagged proteins. Empty backbone for LIC cloning. |
| 193863 | 1BR9-Lifeact | E. coli expression vector for N-ter GFP11 and C-ter R9 tagged lifeact peptide. |
| 193864 | 1BR9-mCherry | E. coli expression vector for N-ter GFP11 and C-ter R9 tagged mCherry. |
| 193865 | 1BR9-AtWUS | E. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana. |
| 193866 | 1BR9-mCherrySTOP | E. coli expression vector for N-ter GFP11 tagged mCherry. |
| 193867 | 1B-GFP1-10 | E. coli expression vector for Hisx6 tagged GFP1-10. |
| 202053 | 1BR9-AtWUSSTOP | E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL |
| 202054 | 1BR9-BFP | E. coli expression vector for N-ter GFP11 and C-ter R9 tagged tagBFP. |
| 202055 | 1BR9-BFPSTOP | E. coli expression vector for N-ter GFP11 R9 tagged BFP |
| 202056 | 1BR9-AtWUSSTOP-Δα3 | E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted. |