A general method for the development of multicolor biosensors with large dynamic ranges.

Hellweg L, Edenhofer A, Barck L, Huppertz MC, Frei MS, Tarnawski M, Bergner A, Koch B, Johnsson K, Hiblot J
Nat Chem Biol. 2023 Jun 8. doi: 10.1038/s41589-023-01350-1. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
193799	pET-51b(+) ChemoG1	Protein production of ChemoG1 in bacteria
193800	pET-51b(+) ChemoG2	Protein production of ChemoG2 in bacteria
193801	pET-51b(+) ChemoG3	Protein production of ChemoG3 in bacteria
193802	pET-51b(+) ChemoG 3.1	Protein production of ChemoG3.1 in bacteria
193803	pET-51b(+) ChemoG 3.2	Protein production of ChemoG3.2 in bacteria
193804	pET-51b(+) ChemoG4	Protein production of ChemoG4 in bacteria
193805	pET-51b(+) ChemoG5	Protein production of ChemoG5 in bacteria
193806	pCDNA5/FRT-ChemoG1	CMV overexpression of ChemoG1 in mammalian cell lines
193807	pCDNA5/FRT-ChemoG5	CMV overexpression of ChemoG5 in mammalian cell lines
193808	pCDNA5/FRT-ChemoB	CMV overexpression of ChemoB in mammalian cell lines
193809	pCDNA5/FRT-ChemoC	CMV overexpression of ChemoC in mammalian cell lines
193810	pCDNA5/FRT-ChemoY	CMV overexpression of ChemoY in mammalian cell lines
193811	pCDNA5/FRT-ChemoR	CMV overexpression of ChemoR in mammalian cell lines
193812	pET-51b(+) ChemoB-CaM	Protein production of ChemoB-CaM in bacteria
193813	pET-51b(+) ChemoC-CaM	Protein production of ChemoC-CaM in bacteria
193814	pET-51b(+) ChemoY-CaM	Protein production of ChemoY-CaM in bacteria
193815	pET-51b(+) ChemoR-CaM	Protein production of ChemoR-CaM in bacteria
193816	pCDNA5/FRT-ChemoG-CaM	CMV overexpression of ChemoG-CaM in mammalian cell lines
193817	pAAV2-hSyn1-NES-ChemoG-CaM	Expression of ChemoG-CaM in primary neurons
193818	pCDNA5/FRT-ChemoG-ATP	CMV overexpression of ChemoG-ATP in mammalian cell lines
193819	pCDNA5/FRT-ChemoB-ATP	CMV overexpression of ChemoB-ATP in mammalian cell lines
193820	pCDNA5/FRT-ChemoR-ATP	CMV overexpression of ChemR-ATP in mammalian cell lines
193821	pCDNA5/FRT-ChemoG-NAD	CMV overexpression of ChemoG-NAD in mammalian cell lines
193822	pCDNA5/FRT-ChemoG-NAD-NLS3x	CMV overexpression of ChemoG-NAD in the nucleus of mammalian cell lines
193823	pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD	CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines
193824	pCDNA5/FRT-ChemoB-NAD	CMV overexpression of ChemoB-NAD in mammalian cell lines
193825	pCDNA5/FRT-ChemoR-NAD	CMV overexpression of ChemoR-NAD in mammalian cell lines
193826	pCDNA5/FRT-ChemoD-NAD	CMV overexpression of ChemoD-NAD in mammalian cell lines
193827	pCDNA5/FRT-ChemoL-NAD	CMV overexpression of ChemoL-NAD in mammalian cell lines
193828	pCDNA5/FRT/TO-ChemoL-NAD-NLS3x	CMV overexpression of ChemoL-NAD in the nucleus of mammalian cell lines
193829	pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD	CMV overexpression of ChemoL-NAD in the mitochondria of mammalian cell lines
193830	pCDNA5/FRT-ChemoL-CaM	CMV overexpression of ChemoL-CaM in mammalian cell lines
193831	pCDNA5/FRT-ChemoL-ATP	CMV overexpression of ChemoL-ATP in mammalian cell lines