Optogenetic polymerization and assembly of electrically functional polymers for modulation of single-neuron excitability.
Sessler CD, Zhou Y, Wang W, Hartley ND, Fu Z, Graykowski D, Sheng M, Wang X, Liu J
Sci Adv. 2022 Dec 9;8(49):eade1136. doi: 10.1126/sciadv.ade1136. Epub 2022 Dec 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193796 | pAAV-CAG-miniSOG-T2A-dTomato | Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato |
| 193798 | pAAV-CAG-SuperNovaGreen-T2A-dTomato | Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato |
| 193913 | pAAV-CAG-miniSOG | Control plasmid that expresses non-targeted miniSOG in mammalian cells |
| 193914 | pAAV-CAG-miniSOG-C-Far | Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells |
| 193915 | pAAV-hSyn-mCherry-T2A-miniSOG-C-Far | Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production |